2025-04-07 作者：wff 来源：文献：
刀豆球蛋白A诱导的葡聚糖纳米凝胶高层级自组装
文献链接：
https://gngfzxb.ecust.edu.cn/cn/article/doi/10.14133/j.cnki.1008-9357.20181124001
作者：
XU Yuan, LIU Lingshan, WANG Hao, DOU Hongjing,
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原文摘要：
A dextran nanogel (Dex NG) with cross-linked structure was prepared through a grafting copolymerization induced self-assembly (GISA) strategy using water-soluble polysaccharide dextran as the main component. GISA is a method that combines free radical grafting polymerization and self-assembly into one step, and can realize large-scale preparation of nanogels. By using cerium ammonium nitrate (CAN) to create free radicals on dextran, methyl acrylate (MA) was initiated to polymerize at the site of free radical to form a grafting copolymer, and a subsequent formation of nanogel was induced by hydrophobic force originated from the resulting MA. Finally, diallyl disulfide (DADS) was added as a cross-linker to stablize the structures. The high order self-assembled nano-aggregates (Con A-Dex NG) were fabricated by Dex NG and concanavalin A (Con A) on the basis of the specific recognition between Con A and the glucose unit in dextran. The particle size, structure and morphology of the self-assembled nano-aggregates were characterized by transmission electron microscopy (TEM), dynamic laser light scattering (DLS), Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance spectroscopy (NMR) and isothermal titration calorimetry (ITC). The mechanism of high order self-assembly was also discussed. In addition, the cytotoxicity of free Con A and Con A-Dex NG on A549 cell (human lung cancer cell) was investigated. The results showed that the size of Con A-dextran nano-aggregates was directly related to the mass ratio of dextran nanogel to Con A. Moreover, the cytotoxicity experiments demonstrated that free Con A could inhibit A549 cells, and its biological activity did not show obvious variations during the process of high order self-assembly.
葡聚糖（Dextran）分子结构特性，利用刀豆蛋白A（Concanavalin A，简称Con A）与葡聚糖分子链中葡萄糖单元（Glucose Units）之间高度特异的分子识别机制，构建了一种具有复杂有序结构的高阶自组装纳米聚集体体系，即Con A-Dex NG（Concanavalin A-Dextran Nanogels，刀豆蛋白A-葡聚糖纳米凝胶）。
图为：TRITC-ConA结构式
以水溶性多糖葡聚糖为主要成分，通过接枝共聚诱导自组装（GISA）策略制备了具有交联结构的葡聚糖纳米凝胶（Dex-NG）。GISA是一种将自由基接枝聚合和自组装结合为一步的方法，可以实现纳米凝胶的大规模制备。利用硝酸铈铵（CAN）在右旋糖酐上产生自由基，引发丙烯酸甲酯（MA）在自由基位置聚合形成接枝共聚物，并在所得MA产生的疏水力的作用下形成纳米凝胶。加入二烯丙基二硫醚（DADS）作为交联剂以稳定结构。
通过透射电子显微镜（TEM）、动态激光散射（DLS）、傅立叶变换红外光谱（FT-IR）、核磁共振光谱（NMR）和等温滴定量热法（ITC）对自组装纳米聚集体的粒径、结构和形态进行了表征。研究了游离Con A和Con A-Dex NG对A549的细胞Poison性。结果表明，Con A-葡聚糖纳米聚集体的大小与葡聚糖纳米凝胶与Con A的质量比直接相关。实验表明，游离的Con A可以抑制A549细胞，其生物活性在高阶自组装过程中没有明显变化。
该Con A-Dex NG纳米聚集体体系在生物医学领域展现出应用前景，例如作为药物递送载体实现靶向给药与控释、作为生物传感器提高检测灵敏度与选择性，以及作为组织工程支架促进细胞黏附与增殖等。其设计理念与制备方法为开发新型生物相容性纳米材料提供了重要参考，推动了纳米技术与生物医学的深度融合与创新发展。
