2024-12-19 作者：ws 来源：文献：
Dual-encapsulated biodegradable 3D scaffold from liposome and waterborne polyurethane for local drug control release in breast cancer therapy
文献链接：
https://www.semanticscholar.org/paper/Dual-encapsulated-biodegradable-3D-scaffold-from-in-Yin-Du/2a9fe264f19550ec37f38cff3b6e7d1044079209
作者：
Hang Yin , Bohong Du , Yue Chen , Nijia Song , Zhen Li , Jiehua Li , Feng Luo &Hong Tan
相关产品：
原文摘要：
Compared with the traditional chemotherapy by injection, local release of drugs in the lesion area is a more efficient and less harmful treatment for solid tumors. However, the selection of appropriate drug carrier and controlled release of chemotherapy drugs are still great challenges. Herein, a kind of dual-encapsulated three-dimensional (3D) scaffold is designed for local drug release via blending the paclitaxel (PTX) loaded phospholipid liposomes with waterborne polyurethane (PU) by freeze-drying. The controlled release of paclitaxel is carried out through two simultaneous procedures. First, liposomes encapsulated in polyurethane scaffold can slowly release by water absorption and degradation of polyurethane. Then paclitaxel encapsulated in liposomes can also be released into water. Compared with the polyurethane scaffold which directly encapsulated paclitaxel, dualencapsulated scaffold has slower initial release amount and maintain higher concentration of paclitaxel in later stage. Moreover, the protection of the phospholipid layer can prevent paclitaxel from being quickly decomposed and cleared, which could greatly improve the bioavailability and therapeutic effect of paclitaxel.Cell experiment results can be seen that dual-encapsulated scaffold not only has higher inhibition rate to the breast cancer MCF7 cells, but also has less damage to normal tissue cells. It provides a more effective platform for the local drug therapy in the treatment of tumors.
DSPE - PEG - FITC 的化学名称是 1,2 - 二硬脂酰 - sn - 甘油 - 3 - 磷酸乙醇胺 - 聚乙二醇 - 异硫氰酸荧光素。它由三个主要部分构成。DSPE（1,2 - 二硬脂酰 - sn - 甘油 - 3 - 磷酸乙醇胺）是一种磷脂成分，含有两条长链的硬脂酸，这使得它具有很强的亲脂性，可以插入脂质双层结构中。PEG（聚乙二醇）的存在增加了整个分子的亲水性，并且能够起到空间位阻的作用，减少在体内与非特异性蛋白的相互作用，其长度可以根据实际需求进行选择，在这里它作为连接 DSPE 和 FITC 的桥梁。FITC（异硫氰酸荧光素）是一种常用的荧光染料，具有明亮的荧光，在波长 490 - 495nm（蓝光）激发下，能发射出 515 - 520nm（绿光）的荧光，用于标记和检测。该文献基于DSPE-PEG-FITC的性质，制备了双封装的PU-PTXLIPO支架，合成过程如下：
图：制备示意图
双封装的PU-PTXLIPO支架的制备：
加入一定浓度的乳液和一定体积比的脂质体，形成一组混合乳剂。然后将混合物乳剂倒入玻璃皿中，然后放入烤箱中浸泡，蒸发水。采用衰减全内反射傅里叶变换红外光谱（ATRFTIR）和x射线光电子能谱（XPS）测量PU中的脂质体。将DSPE-PEG-FITC和卵磷脂按一定摩尔比混合制备荧光脂质体，然后将荧光脂质体加入PU膜和支架中。采用共聚焦激光扫描显微镜观察荧光图像。
图：粒径分布图
结论：
该文献成功地制备了双封装的PU-PTXLIPO支架。这种支架的化合物释放特性比PU-TAXOL支架更温和、更稳定，脂质体的加入可以保护紫杉醇在细胞基质中的降解，延长其释放过程中的使用寿命，提高了利用率。这种具有可控释放作用的支架可以减少对正常细胞的初始损伤，维持体外对tumour细胞的有效抑制。
