文献：Efficient Lung Cancer-Targeted Drug Delivery Via a Nanoparticle/MSC System

文献链接：https://www.cqvip.com/doc/journal/2718115194

作者： Wang, XushengChen, Haiyaneng, XiaoweiGuo, WenpengJin, YuWang, ShanTian, RuiyunHan, YanjiangGuo, Ling,Han, JiminWu, Yaojiong,Mei, Lin

相关产品：Cy5-PEG-NH2 花菁染料CY5-聚乙二醇-氨基

原文摘要：Low targeting efficiency limits the applications of nanoparticles in cancer therapy. The fact that mesenchymal stem cells (MSC) trapped in the lung after systemic infusion is a disadvantage for cell therapy purposes. Here, we utilized MSC as lung cancer-targeted drug delivery vehicles by loading nanoparticles (NP) with anti-cancer drug. MSC showed a higher drug intake capacity than fibroblasts. In addition, MSC showed predominant lung trapping in both rabbit and monkey. IR-780 dye, a fluorescent probe used to represent docetaxel (DTX) in NP, delivered via MSC accumulated in the lung. Both in vitro MSC/A549 cell experiments and in vivo MSC/lung cancer experiments validated the intercellular transportation of NP between MSC and cancer cells. In vivo assays showed that the MSC/NP/DTX drug delivery system exerted primary tumor inhibition efficiency similar to that of a NP/DTX drug system. Collectively, the MSC/NP drug delivery system is promising for lung-targeted drug delivery for the treatment of lung cancer and other lung-related diseases. (C) 2019 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

该文献结合纳米技术和干细胞技术，将荧光染料Cy5-PEG-NH2与纳米粒子结合，并通过干细胞作为载体，将化合物准确传输到lung cancer细胞中。通过采用纳米技术，将化合物包裹在纳米粒子中，实现化合物的靶向输送，并提高化合物的吸收率和效果。并详细介绍了其制备过程。

图为：靶向传递载体

   共聚物PLGA-b-PEG的合成方法。简而言之，用DCC(NHS和DCC和PLGA在室温下活化24小时。通过过滤去除反应副产物二环己基碳二尿素（DCU），将活化的PLGA（PLGA-NHS）在无水醚中沉淀，用甲醇过滤和洗涤。PLGA-NHS在真空中干燥24小时。将PLGA-NHS溶解于无水二氯甲烷中。然后，在磁性搅拌下加入M-PEG-NH2。反应在干燥的氩气气氛下进行12 h，所得溶液在冷无水醚中沉淀。将沉淀后的共聚物PLGA-b-PEG进行过滤，用甲醇洗涤，最后在真空下干燥24 h。此外，荧光标记共聚物PLGA-b-PEG-FITC和PLGA-b-PEG-Cy5。

结论：研究证明，Cy5-PEG-NH2在lung cancer靶向化合物传递中具有作用。通过构建纳米颗粒和间充质干细胞（MSC）系统，cy5-PEG-NH2能够有效地靶向lung cancer细胞并释放化合物。实验结果显示，cy5-PEG-NH2可以增强化合物在tumour组织中的积累，提具有效果，并减少对正常组织的损伤。此外，cy5-PEG-NH2还表现出良好的生物相容性和稳定性。