文献：NIR Laser Responsive Nanoparticles for Ovarian Cancer Targeted Combination Therapy with Dual-Modal Imaging Guidance

文献链接：https://www.dovepress.com/nir-laser-responsive-nanoparticles-for-ovarian-cancer-targeted-combina-peer-reviewed-fulltext-article-IJN

作者：Jiawen Zhao，Liang Zhang，Yingjie Qi，Kui Liao，Zhigang Wang， Ming Wen，Di Zhou

相关产品：

Bi2S3 modified by oleic acid  油酸修饰的硫化铋

PLGA-PEG-FA（聚乳酸-羟基乙酸共聚物-聚乙二醇-叶酸）

原文摘要：

Purpose: Multifunctional nanoparticles with targeted therapeutic function and diagnosticimaging are of great interest in the domain of precision therapy. NIR laser responsive nanoparticles (PLGA-PEG-FA encapsulating Bi2S3, PFP, and Dox (designed as FBPD NPs)) are synthesized for ovarian cancer targeted combination therapy with CT/PA dualmodal imaging guidance (PA: photoacoustic; CT: X-ray computed tomography).

Methods and Results: The FBPD NPS prepared by the double emulsification method revealed excellent dispersity, great stability, outstanding optical properties. The temperature of FBPD NPs increased rapidly after laser irradiation, inducing liquid-to-gas conversion of perfluoropentane (PFP), and promoting the release of Dox up to 86.7%. These FBPD NPs demonstrated their outstanding imaging capability for both PA and CT imaging both in vitro and in vivo, providing the potential for therapeutic guidance and monitoring. Assisted by folic acid, these nanoparticles could highly enrich in ovarian tumor tissue and the accumulation peaked at 3 h after intravenous administration. The desirable photothermal-conversion efficiency of the nanoparticles combined with chemotherapy achieved highly efficient therapy, which was demonstrated both in vitro and in vivo.

Conclusion: We successfully constructed multifunctional theranostic FBPD NPs for highly efficient PTT/chemotherapy combined therapy with dual CT/PA imaging guidance/monitoring. The unique nanoparticles with multiple abilities pave an emerging way toward precise treatment of ovarian cancer.

Bi2S3 modified by oleic acid：油酸（oleic acid）修饰的硫化铋（Bi2S3）是一种将硫化铋的特性与油酸的表面修饰作用相结合的材料。硫化铋是一种具有独特光学和电学性质的半导体材料，而油酸是一种长链脂肪酸，可用于修饰硫化铋的表面。

PLGA-PEG-FA：PLGA 是聚乳酸 - 羟基乙酸共聚物。它是由乳酸（Lactic Acid）和羟基乙酸（Glycolic Acid）通过缩聚反应生成的。乳酸有 L - 乳酸和 D - 乳酸两种异构体，其比例不同会影响 PLGA 的性能。PEG 是聚乙二醇（Polyethylene Glycol），是一种具有良好亲水性的聚合物。它的分子链含有大量的醚键（-O-），这使得 PEG 具有出色的水溶性。FA 是叶酸（Folic Acid）。叶酸是一种维生素，在人体的新陈代谢过程中发挥着重要作用。在这个共聚物中，叶酸作为靶向分子，它能够与细胞表面的叶酸受体特异性结合，尤其是在一些tumour细胞表面，叶酸受体呈现高表达状态。基于Bi2S3 modified by oleic acid、PLGA-PEG-FA的性能，NIR激光响应纳米颗粒FBPD NPs的合成如下：

图：合成示意

   FBPD NPs的合成：

   将阿霉素溶解在双蒸馏水中；然后在上述溶液中加入 PFP，然后在冰浴下进行探针超声处理。将Bi2S3和PLGA-PEG-FA溶解在CHCl2中，并与之前制备的混合物混合。立即，用超声波探针将该化合物乳化，形成初始乳化液。对于第二种乳液，将PVA加入到初始乳液中，通过探针超声混合。随后，将第二乳液稀释在异丙醇溶液中，将制备的乳液冰浴搅拌，使CHCl2完全挥发。最后，在离心后收集FBPD NPs。至于FBPNPs或BPD NPs的制备方法，区别在于去除FA或Dox。所有的行动都是在黑暗中进行的。

图：表征图像

结论：

该文献成功制备基于Bi2S3 modified by oleic acid、PLGA-PEG-FA合成的NIR激光响应纳米颗粒FBPD NPs。数据显示，FBPD NPs在体外和体内都显示了其出色的PA和CT成像的成像能力，为heal 指导和监测提供了潜力。纳米颗粒结合光热转换效率实现了效果，这在体内和体外都得到了证明。