mPEG-COOH在超灵敏生物检测的应用
瑞禧生物2025-06-19   作者:wff   来源:
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文献:Core-satellite assemblies and exonuclease assisted double amplification strategy for

ultrasensitive SERS detection of biotoxin

文献链接:https://www.cqvip.com/doc/journal/2714144957

作者:Dandan Huang, Jiaming Chen, Li Ding, Longhua Guo, Palanisamy Kannan, Fang

Luo, Bin Qiu, Zhenyu Lin

相关产品:mPEG-COOH 甲氧基聚乙二醇-羧基

原文摘要:In this work, core-satellite assemblies and exonuclease assisted double amplification strategy is developed to produce surface-enhanced Raman scattering (SERS) biosensor towards ultrasensitive detection of biotoxin. In the presence of target molecules, the exonuclease III (Exo III) assisted efficient recycling amplification provides an excellent pathway for the fabrication of core-satellite SERS sensor. Briefly, the proposed strategy includes the following double amplifications: (i) Exo III induced target-related signal amplification;(ii) core-satellite assemblies assisted formation of SERS "hot-spots" induced signal amplification. To show the applicability of the suggested strategy, the detection of ochratoxin A (OTA), one of the most toxic and widely distributed biotoxin, is demonstrated as an example. The results show that the limit of detection (LOD) of OTA is 0.83 fg mL(-1) (S/N = 3). On the basis of the DNA aptamer induced specific target recognition, hence our sensing strategy is easy to be expended to the ultrasensitive detection of other targets, e.g., DNAs, RNAs, and other molecules that have corresponding DNA aptamers. (C) 2020 Elsevier B.V. All rights reserved.

 

本篇论文研究了mPEG-COOH在Core-Satellite组装和外切酶辅助双重扩增策略中的关键作用,推动超灵敏SERSbiotoxin检测并探讨了mPEG-COOH在Core-satellite组装以及外切酶辅助双重扩增策略中的应用,提升了表面增强拉曼散射(SERS)技术在biotoxin检测中的灵敏度。该研究的进展为生物检测领域开辟了新的方向,特别是在快速、灵敏地检测有害biotoxin方面具有重要意义。

           25 nm Au NPs的(A) TEM图像

图1:25 nm Au NPs的(A) TEM图像。

在本研究中,mPEG-COOH作为一种重要的改性分子,发挥了重要的作用。首先,mPEG-COOH被用作Core-satellite组装中的连接分子,能够有效地通过其羧基与金纳米粒子表面结合,从而增强纳米粒子之间的相互作用,形成高度有序的组装结构。这种结构不仅提高了SERS信号的强度,还大幅度扩大了检测目标分子时的选择性。

 

其次,mPEG-COOH在外切酶辅助双重扩增策略中也发挥了重要作用。通过其化学特性,mPEG-COOH能够有效地与外切酶反应,促进目标分子的扩增。这一过程提高了检测灵敏度,将biotoxin的检测限降低到了前所未有的水平,为快速的toxin检测提供了理论依据。

检测 

图2:(A)OTA检测的优化(B)反应时间(C)和Au NPs核心卫星组装形成时间的影响。

 

 

结论:mPEG-COOH作为核-卫星结构中的核部分,通过与金纳米棒的修饰,形成了核饼图形状的核-卫星结构。该结构不仅可以提供高表面增强拉曼散射(SERS)效应,还可以具有生物兼容性和稳定性。此外,mPEG-COOH还引入了多个-COOH功能基团,可以用于进一步的修饰和接触链。通过将biotoxin与mPEG-COOH修饰的金纳米棒结合,可以实现对biotoxin的高灵敏度检测。