文献：Dynamic-Covalent Hydrogel with NIR-Triggered Drug Delivery for Localized Chemo-Photothermal Combination Therapy

文献链接：https://xueshu.baidu.com/usercenter/paper/show?paperid=1t2y0gg0dv700aq0xk0s0ts0c5671192&site=xueshu_se

作者：Pengfei Sun,Ting Huang, Xiaoxiao Wang,Gaina Wang,Zhijia Liu,Guosong Chen,and Quli Fan

相关产品：PF127

原文摘要：Near-infrared (NIR) light-responsive, injectable hydrogels are among the most promising drug delivery systems for localized anticancer therapy owing to its minimally invasive

administration and remote-controlled manner. However, most currently reported NIRresponsive hydrogels were usually generated through physical mixing of thermosensitive polymers and photothermal conversion agents. In this study, a novel type of dynamic-covalent hydrogel (GelPV-DOX-DBNP) with NIR light-triggered drug release behavior was rationally designed for chemo-photothermal combination treatment of tumors. Concretely, this NIRresponsive hydrogel was formed by specific benzoxaborole-carbohydrate interactions between benzoxaborole (BOB)-modified hyaluronic acid (BOB-HA) and fructose-based glycopolymer(PolyFru), where photosensitizer perylene diimide zwitterionic polymer (PDS), reductant ascorbic acid (Vc), anticancer drug doxorubicin (DOX) as well as photothermal nanoparticles (DB-NPs) were encapsulated, simultaneously. Upon 660 nm light irradiation, both PDS and Vc within the designed hydrogel can convert oxygen into hydrogen peroxide, which could make hydrogel be degraded through the breakage of dynamic covalent bonds based on

benzoxaborole-carbohydrate interactions, leading to NIR light-activatable release of DOX and DB-NPs from GelPV-DOX-DBNP. Furthermore, the released DB-NPs can convert 915 nm light irradiation into heat, enabling the application of GelPV-DOX-DBNP as a NIR-responsive drug delivery platform for both chemotherapy and photothermal therapy (PTT). In vivo results prove that GelPV-DOX-DBNP exhibited a markedly enhanced chemo-photothermal synergistic therapy for 4T1 tumor model mice, compared to alone chemotherapy or PTT. This work presents a new strategy to construct NIR light-responsive hydrogel as one alternative drug delivery system for anticancer applications.

近红外（NIR）光响应、可注射水凝胶是局部给药系统之一。然而，目前报道的大多数NIR反应性水凝胶通常是通过热敏聚合物和光热转化剂的物理混合而产生的。设计一种具有NIR光触发化合物释放行为的新型动态共价水凝胶（GelPV-DOX-DBNP）。这种NIR响应性水凝胶是由苯并恶硼（BOB）修饰的透明质酸（BOB-HA）和果糖基糖共聚物之间特定的苯并恶硼-碳水化合物相互作用形成的（PolyFru），其中光敏剂过炔二亚胺两性离子聚合物（PDS）、Vc、阿霉素（DOX）以及光热纳米颗粒（DB-NPs）同时被封装。

图：由PDPPBT和PF127自组装的DB-NPs

DB-NPs的制备

在THF中溶解制备PDPPBT溶液，在蒸馏水中制备PF127溶液。将PDPPBT与PF127溶液的体积比保持在一定比例，然后在超声处理下将PDPPBT溶液快速倒入PF127水溶液中。随后，THF在通风室中搅拌蒸发完全去除。最后，使用管状超滤模块去除多余的PF127，然后将DB-NPs溶液浓缩。

图：DOX和DB-NPs的NIR光触发释放行为示意图

结论：在660 nm光照射下，设计的水凝胶中PDS和Vc都可以将氧转化为过氧化氢，使水凝胶通过苯并恶硼-碳水化合物的动态共价键相互作用而降解，导致NIR光激活释放GelPV-DOX-DBNP的DOX和DB-NPs。PF127参与制备的DB-NPs可以将915 nm的光照射转化为热，使GelPV-DOX-DBNP作为NIR响应的化合物传递平台用于Chemotherapy 和光热（PTT）。